Applied Ecology Specialization in
Natural Resource Sciences Graduate Program

Available to MS and PhD candidates.

Students in the Applied Ecology specialization focus on the interactions among ecosystem components. This specialization is designed for students interested in applying ecological principles to the management of terrestrial and aquatic ecosystems.

The School of Natural Resources is an academic unit in the Institute of Agriculture and Natural Resources at the University of Nebraska-Lincoln. Most faculty and graduate students in the School reside in Hardin Hall on UNL’s East Campus. The building has excellent laboratory, office, and classroom facilities to support research, teaching and outreach activities.

Faculty members within the Applied Ecology specialization have strong cooperative efforts with other departments and colleges. Graduate students seeking a MS or PhD specialization in Applied Ecology from SNR will find a variety of options available to help tailor the program to their unique interests.

Educational goals and objectives:

  1. Provide students a learning environment to promote a broad understanding of ecological principles and how they can be applied to the conservation and management of our natural resources.
  2. Recognize students who have attained an advanced knowledge of ecological science and management.
  3. Support interactions and cooperation among the community of scientists and students working on applied ecological problems.

Scope:

While there are no specific guidelines or national accreditation programs, students will be encouraged to seek a broad background in several areas focusing on interactions among ecosystem components. The specialization is designed for students interested in applying ecological principles to the management of terrestrial and aquatic ecosystems. The specialization should appeal to students interested in

  • agroforestry/agroecology
  • aquatic ecology
  • fisheries ecology
  • stream ecology
  • conservation biology
  • forest ecology
  • grassland ecology
  • wildlife ecology
  • ecosystem science

Admission Requirements

Please see our admission requirements and application procedure pages for more information about our admission and application expectations for graduate degree candidates.

Graduation Requirements

Master of Science

  • Thirty (30) hours of graduate courses are required for the degree.
  • Individualized programs are developed by the student and their supervisory committee in accordance with the requirements of the M.S. in Natural Resource Sciences degree as detailed in the Graduate Studies Bulletin.  Students are encouraged to take a broad array of classes that will provide the background to address ecosystem issues from various perspectives.
  • Student must complete a thesis research topic in Applied Ecology.
  • Student must complete NRES 801 (2 cr).
  • The chair (or one co-chair) of the student’s supervisory committee must be a faculty member from the Applied Ecology Group and a member of the UNL graduate faculty.
  • At least two courses of the program must be “NRES” courses or a substitute course approved by the Applied Ecology faculty. 

Doctor of Philosophy

  • Ninety (90) hours of graduate courses are required for the degree.
  • Individualized programs are developed by the student and their supervisory committee in accordance with the requirements of the Ph.D. in Natural Resource Sciences degree as detailed in the Graduate Studies Bulletin.  Students are encouraged to take a broad array of classes that will provide the background to address ecosystem issues from various perspectives.
  • Student must complete a dissertation research topic in Applied Ecology.
  • Student must complete NRES 801 (2 cr).
  • The chair (or one co-chair) of the student’s supervisory committee must be a faculty member from the Applied Ecology Group and a member of the UNL graduate faculty.
  • At least three courses of the program must be “NRES” courses or a substitute course approved by the Applied Ecology faculty. 

Career Opportunities

The specialization should appeal to students interested in academic, state/federal agency, NGO, and professional careers in

  • agroforestry/agroecology
  • aquatic ecology
  • fisheries ecology
  • stream ecology
  • conservation biology
  • forest ecology
  • grassland ecology
  • wildlife ecology
  • ecosystem science
deer tagging
Student with fish

Selected Dissertations & Theses

Ecological Impacts of Restoring Fire-Grazing Interaction in Sandhills Prairie through Patch-Burn Grazing - Nolan Sipe
  • Thesis Defense
  • 11/30/2023
A Collaborative Adaptive Management (CAM) Project was started at the University of Nebraska in 2020 to address some of key uncertainties related to the management of grasslands in the Nebraska Sandhills through stakeholder driven experiments and the adaptive management cycle. Patch-burn grazing was selected by CAM as a management tool to generate heterogeneity across the landscape and promote biodiversity while balancing economic and ecological trade-offs. The patch-burn grazing system was implemented with controlled burns in May of 2022 and March of 2023. The goal of the research present here is to understand how the use of patch-burn grazing impacts several ecological aspects of the Nebraska Sandhills. Specifically, this study asks three things 1) does patch-durn grazing negatively impact soil conditions by increasing soil erosion and depleting the thin topsoil of nutrients in burned areas, 2) is patch-burn grazing able to significantly change vegetation structure and promote vegetation heterogeneity, and 3) how does patch-burn grazing affect avian communities, abundance, and species diversity in the Sandhills? Comparison of soil conditions between burned and unburned was made using the change of soil height at installed erosion pins and a series of soil nutrient panels samples taken in the months following the fire. Vegetation structural metrics and function group covers were measured throughout each field in the study. A nonmetric multidimensional scaling (NMDS) ordinal analysis supported by a pairwise comparison using permutational multivariate analysis of variance (PERMNOVA) was used to examine the difference in vegetation structure between each of the fields in this study. Bird point counts were performed in each field throughout the two years of this study. This data was used to calculate and compare species diversity, community, and the abundance of a few select species. NMDS and PERMNOVA were used to explore the difference between fields in the composition of the avian communities, while N-mixture models were used to estimate abundance. This study provides an understanding of how patch-burn grazing, an under-utilized tool in the Nebraska Sandhills, can be used to support a more heterogeneous and resilient grassland.
Precipitation and Greenness in Pastoral Lands of East Turkana, Kenya - Paul Akpejeluh
  • Thesis Defense
  • 11/21/2023
Pastoralism has long supported livelihoods and provided essential ecosystem services in landscapes of East Africa. Vegetation productivity is central to the functioning of pastoral systems but may be affected by changes in climate and land use. Vegetation monitoring is important for understanding the effects of global change in pastoral lands; however, it can be time and resource intensive. Remote sensing provides opportunities for efficient multi-scale monitoring of vegetation and climatic drivers. In this thesis, I explore the utility of satellite and UAV remote sensing for monitoring vegetation and precipitation trends and relationships in the East of Lake Turkana Region of northern Kenya. In Chapter 1, I examine regional greenness and precipitation time series at monthly, seasonal, and annual temporal resolutions, as well as relationships between greenness and precipitation from 2000 to 2022. I found evidence of long-term precipitation–greenness coupling at monthly and annual temporal resolutions. There were no trends in monthly or annual regional precipitation, while NDVI significantly increased at monthly temporal resolution but did not exhibit a significant trend at annual temporal resolution. Traditional pastoral practices, such as use of livestock corrals (bomas), also influence local vegetation composition and abundance. In chapter two, I use satellite and unmanned aerial vehicle (UAV) remote sensing data to monitor greenness in and around abandoned boma settlements at seasonal and annual temporal resolutions. Results showed that mean NDVI from UAV and Sentinel-2 data varied based on seasons (dry or wet) and from boma to boma. NDVI significantly differed between bomas and non-boma sites and there was significant positive correlation between NDVI and precipitation across all bomas, with an optimum temporal lag response of one month. Collectively, my results add to the body of literature demonstrating the utility of satellite and UAV-based remote sensing data for monitoring vegetation in pastoral systems.
Population Demography of a Glacial-Relict Stream Fish Mediated via Anthropogenic Alteration - Joseph Spooner
  • Thesis Defense
  • 11/15/2023
Northern Pearl Dace Margariscus nachtriebi are a small-bodied glacial-relict fish species of greatest conservation need (SGCN) found throughout Canada and the northern U.S.A. Their distribution within the Nebraska Sandhills Ecoregion is isolated from the northern core distribution of the species following the last glaciation period approximately 18,000 years ago. Headwater streams within the Nebraska Sandhills Ecoregion are predominately groundwater fed and provide the cool water temperatures needed to support Northern Pearl Dace and other glacial-relict SGCN. Headwater streams within the Nebraska Sandhills Ecoregion have been geomorphically altered through anthropogenic processes such as channelization whereby habitat homogenization has occurred. Evidence of stream habitat changes stemming from channelization directly influencing fish population demographic parameters is limited. Capture-mark-recapture studies used to estimate population demographic parameters may provide insight into the linkages between stream habitat alteration and influences on demographic parameters of fishes. However, limited methods exist to individually mark small-bodied fish <100mm that do not alter behavior or reduce survival of marked individuals. Here, we investigated 1) small-bodied fish survival and tag retention using p-Chip microtransponder tags, and 2) annual survival of Northern Pearl Dace in channelized stream sites within the Nebraska Sandhills Ecoregion. We found tag retention of p-Chip microtransponder tags was high in small-bodied fish and did not affect fish survival. Northern Pearl Dace annual survival significantly differed between channelized stream sites and non-channelized stream sites. These results highlighted the utility of p-Chip microtransponder tags as effective marks for use in small-bodied fish where individual identification is needed. Further, these results indicate channelization may reduce Northern Pearl Dace survival when assuming complete site fidelity. As such, extensive channelization practices within the Nebraska Sandhills Ecoregion may ultimately influence distribution patterns of Northern Pearl Dace populations. Management efforts to increase Northern Pearl Dace populations may benefit from mitigation of channelized streams. Further research assessing spatiotemporal responses of Northern Pearl Dace to channel restoration practices may further refine specific habitat manipulation techniques and spatial distribution of habitat patches needed within watersheds.
Habitat heterogeneity in Nebraska streams and distribution prediction for tier-1 cyprinids using multi-scale modeling of fluvial and landscape features - Connor Hart
  • Thesis Defense
  • 11/13/2023
Multiscale environmental processes determine in-stream habitat conditions which drive species distributions. Habitat constitutes the physical template upon which ecological processes occur and species conduct life stage activities. Habitat heterogeneity promotes biodiversity of aquatic systems. Stream classification informs freshwater conservation by providing a useful framework to account for habitat heterogeneity, often based on landscape regions of similar environmental processes. A greater understanding of landscape-based classification frameworks as means to classify stream systems may improve understanding of drivers of biodiversity. Using Nebraska as a case study, on a statewide scale, objectives were 1) to characterize habitat availability for several at-risk fish species, and 2) to assess the categorization of stream habitat heterogeneity within multiple classification systems. Species distribution models provide a tool to characterize spatial habitat patterns and quantify species-habitat relations. Species distribution models were constructed from environmental data and historical presence data for 3 at-risk cyprinid species including Flathead Chub, Plains Minnow, and Northern Redbelly Dace. Our results support current knowledge of impactful habitat features based on species traits and environmental processes shaping site-scale habitat conditions. For all 3 species, hydrological factors such as flow velocity and discharge were among the most influential habitat conditions of examined variables. Further, variables representing stream size and position in the drainage network consistently carried high influence. We used site-level stream data collected by the Nebraska Department of Environment and Energy (NDEE) from 1995-2021 to assess stream habitat heterogeneity among Biologically Unique Landscapes (BUL) at a statewide spatial scale. Multivariate cluster analysis and statistical similarity tests were used to determine if aquatic habitat within the BULs is unique and by which features they vary. No significant differences were found between aquatic environmental conditions within and outside BULs. Evidence for unique aquatic habitat conditions among BULs was limited, shown in minimal pairwise differences among BULs in statistical similarity tests. Furthermore, the classificatory power of the BULs underperformed all other classifiers and, as such, BULs may not improve statewide stream classification.
Understanding the Factors Affecting Nebraskan Farmers' and Landowners' Decision to Adopt Precision Agricultural Technologies and Programs - Corrin Winter
  • Thesis Defense
  • 08/07/2023

Agriculture intensification has increased during the past 50+ years resulting in simplified cropping systems (e.g., monocultures of high-yielding crops such as corn and soybeans). Additionally, agricultural intensification has led to the removal of non-crop habitat to maximize field size for production. Concomitantly, agricultural intensification coupled with increased chemical and mechanical inputs has led to adverse environmental impacts on soils, water, and biodiversity. To address these issues, new precision agriculture technologies and conservation planning frameworks have been developed with the goal of optimizing agricultural production systems while reducing negative environmental impacts soils, water, and biodiversity. Despite these opportunities to optimize agricultural production and environmental sustainability, farmers still face many constraints and limitations when choosing to adopt these precision technologies.

To identify the factors that facilitate or constrain farmers and farmland owners’ willingness to participate in targeted conservation delivery (via precis ion agriculture), we surveyed 7,503 individuals across Nebraska. These individuals were selected based on two criteria: 1) they were farmers and/or farmland owners who own or work land in Nebraska; and 2) were over the age of 19. We received 1,040 completed surveys (response rate = 13.9%). Our preliminary results indicate that upfront costs, upkeep costs of equipment, and the right to repair were the most common constraints identified by farmers and farmland owners’ willingness to adopt targeted conservation approaches using new precision agriculture technologies. We also found that inheritance and being a renter had a significant impact on their perceived limitations. Finally, we examined how farmers and farmland owners preferred to receive information about conservation programs across Nebraska. Survey participants preferred receiving information from: 1) farm equipment manufacturers; 2) farm-oriented news networks; and 3) farm service agencies. Non-government organizations (e.g., Pheasants Forever), university extension, Nebraska Natural Resources Districts, and the USDA-Natural Resource Conservation Service were preferred more by applicants who had applied for conservation program compared to those who had never applied for a conservation program. We also examined the perceived sufficiency of the expert advice the respondents received when applying to a conservation program. The study found that the more agencies or organizations the producer worked with, the more likely they were to find the information they received sufficient. Knowledge gained from this study will also help conservation agencies/organizations improve their outreach and engagement methods with Nebraskan farmers and farmland owners with the goal of optimizing agricultural production and environmental sustainability for future generations.

Lay Abstract Version

To improve both environmental conditions and farmer profitability, we delved into the challenges faced by Nebraskan farmers and landowners when adopting new farming technologies. We looked at data from a 2022 survey with 7,503 participants which included farmers and landowners from across the state of Nebraska. Our goal was to uncover why some adopt new precision agricultural and conservation technologies while others don't. Precision agriculture is the use of technology to improve farmland conditions. For example, using a yield monitor to monitor how many crops grow in each area. Precision conservation is similar, however it focuses on improving environmental conditions on the farm, like soil erosion. In addition, our research was designed to help agencies better share information about precision farming and conservation in a way that benefits the people of Nebraska. Our findings highlighted that finances were the main reason many people were hesitant to adopt precision farming tools. Factors like renting land or being a first-generation farmer also affected which factors influence these decisions. People also preferred getting information about precision farming technologies from friends, family, and fellow farmers, rather than government agencies. Interestingly, those who did favor information from non-governmental agencies were more likely to apply for conservation programs. This shows the importance of government agencies working together with non-government organizations and private companies to promote precision farming technologies and practices. Furthermore, our research showed that farmers and landowners were more satisfied with expert advice when they worked with more agencies and companies. As such, we should encourage cooperation between various agencies and organizations. Cooperation is key to fostering positive attitudes toward the adoption of precision agricultural and conservation technologies and practices in Nebraska.

Light alters leaf breakdown rates and metabolic activity in anthropogenically-impacted Nebraska streams - Reilly Farrell
  • Thesis Defense
  • 07/17/2023
Algal growth has been shown to influence carbon breakdown rates in aquatic ecosystems; however, the magnitude and direction of these effects differ based on ecosystem context. To explore algal priming effects on decomposition in anthropogenically-impacted ecosystems, I conducted light manipulation experiments in three streams, each surrounded by different land uses (urban, row crop, and CAFO) across Nebraska. I measured leaf breakdown rates, metabolic activity, fungal and algal biomass, and leaf percent carbon (C) and phosphorus (P) to assess the effect of light differences. Experimental streams were impacted by sedimentation and turbidity over the course of the leaf incubation. Still, I observed significantly higher algal biomass as chlorophyll a in light treatments at two of my three streams. I observed negative relationships between algal biomass and fungal biomass as ergosterol at all my sites but contrasting relationships between algal biomass and respiration rates at my two agricultural sites. Additionally, breakdown rates were not significantly different by light treatment at any of my row crop sites, but they were higher at two of my last three collection dates at the CAFO site. Overall, leaf C:P declined over the course of the experiment. These results support prior studies that light levels can alter breakdown rates of allochthonous material in streams. They shed light on the influence that changing light levels can exert over carbon processing in streams.
Proghorn Survival and Resource Selection in Nebraska’s Agriculturally Dominated Landscape - Katie Piecora
  • Thesis Defense
  • 07/13/2023
Nebraska’s pronghorn population has been stable over the last decade, yet their presence on the landscape remains a contentious subject amongst private landowners. Conversion of grassland for agriculture and increased anthropogenic activity has drastically altered pronghorn behavior throughout their current range, however basic ecology, and resource use by pronghorn in Nebraska remains poorly understood. Establishing baseline population metrics and seasonal patterns of resource use for this population at the eastern periphery of the species range is critical to guide management actions. We deployed GPS collars on 110 adult pronghorn to quantify survival, mortality risk, and seasonal resource selection in western Nebraska. We used Kaplan-Meier and Cox Proportional Hazards modeling approaches to calculate adult survival and mortality risk. We found that annual survival was 0.65, survival significantly decreased in autumn, and mortality risk was 2.36 times higher for males than females. To investigate seasonal resource selection, we used a Step Selection Function approach, which uses conditional logistic regression to compare predictor variables at used and available locations. Seasons were delineated as winter (1 Dec-28 Feb), spring (1 Mar-31 May), summer (1 Jun-31 Aug), and autumn (1 Sept-30 Nov). Used locations showed no movement across Interstate 80 and habitat composition differed on either side so we evaluated resource selection separately between the herds north and south of the interstate. Our models included 2-way interactions with sex to determine if sexual segregation plays a role in selection. We evaluated selection of grassland/herbaceous landcover, agricultural land, human development, distance to water, and distance to roads. Pronghorn selected cropland and avoided human development regardless of herd, sex, or season. Use of agricultural areas was disproportionate to its availability on the landscape. Selection or avoidance of roads and water varied across herd, sex, and season. This work begins the complex task of understanding pronghorn ecology in an agricultural system. Our research aims to provide valuable insight into the factors influencing population dynamics so that biologists can implement effective strategies to manage pronghorn populations in western Nebraska.
Primate social interactions and pathogen shedding in a biodiversity hotspot - Marnee Roundtree
  • Dissertation Defense
  • 07/13/2023

Interconnected wildlife health and behavior play vital roles in population dynamics, ecosystem functions, biodiversity, and wildlife conservation. Research and public interest in zoonotic pathogen transmission -- transmission from animals to humans -- has increased with the COVID-19 pandemic and ongoing concern about zoonotic spillover from wildlife to people. Diverse zoonotic pathogens have been linked to wildlife reservoirs, highlighting the importance of understanding human-wildlife interfaces and interactions. However, humans and domestic animals impact the health and conservation of threatened and endangered species, including primates. Both primate and human behavior can influence the risk of pathogen transmission at the primate-human interface. While zoonotic pathogen sharing is of concern, less research has focused on pathogen transmission among wildlife and integrating social behavior into wildlife health research.

To better understand the links between primate social behavior and pathogen transmission, we used long-term ecological transect data from the Udzungwa Ecological Monitoring Centre and a cross-sectional survey of pathogen shedding in overlapping primate populations living in forests of varying protections status and human disturbance in the Udzungwa mountains of Tanzania. Our research quantifies inter-specific associations among free-ranging primates in two forest blocks of the Udzungwa Mountains to investigate the relationship between forest protection status and inter-specific associations. Using bias-reduced logistic regression and social network analysis, we found significant and varied trends in inter-specific associations in well and less protected forests among primate species. We then examined the relationship between habitat primate inter-specific associations in the well-protected Mwanihana forest. Using mixed model logistic regression and a co-occurrence checkerboard score, we found that habitat and species were significant predictors of inter-specific associations. We found strong inter-specific associations between Udzungwa red colobus (Piliocolobus gordonorum) and Peter’s Angola colobus (Colobus angolensis palliatus) across habitats. To investigate connections between primate social interactions and health, we assessed the relationship between viral shedding and interspecific interactions observed in the field. While the relationship was not significant, the majority of animals with viral shedding detected were sampled at sites with inter-specific associations. These results highlight the need for integrated wildlife behavior and health research to understand connections between primate social interactions and pathogen transmission.

Title: Bat use of afforested and encroached patches and their role in extending bat habitat into the Nebraska Sandhills. - Jacob Wagner
  • Thesis Defense
  • 05/17/2023
The Nebraska Sandhills are currently undergoing a state shift to a redcedar dominated woodland due to anthropologic planting of eastern redcedar (Juniperus virginiana) and woody encroachment from the periphery of the Sandhills. To better understand this novel ecosystem and how bats are utilizing it we collected data at Barta Brothers Ranch with acoustic sensor grids consisting of 24 100m spaced acoustic sensors placed adjacent to planted windbreaks. Supplemental data from the Nebraska North American Bat Monitoring Program was used for data analysis at larger spatial scales. We used Spearman ranked correlation and kriging interpolation maps to see how bats used windbreaks during nightly activity. Eptesicus fuscus, Lasiurus borealis, and Nycticeius humeralis showed close usage pattern to the windbreaks while Lasiurus cinereus and Lasionycteris noctivagans showed usage patterns outside of our sensing area, while still showing a correlation to trees at a larger scale. We used multi model inferencing and model averaging to find the best model to explain bat species richness and call count. We found that distance to trees, with a preference to deciduous, and time through the summer to be the best predictors of bat species richness and call count. We used linear regressions of first instance calls in our grids to determine if bats are using windbreaks to roost or travel from their roost to feeding grounds. All species combined, Eptesicus fuscus and Lasionycteris noctivagans showed higher numbers of first instances closer to the windbreak, this shows that bats, in general, are using windbreaks for roosting or for roads from roost to their feeding grounds. Lasiurus cinereus reported a flat regression line, hinting that this species may travel across open grassland from roost to feeding grounds. Bat presence and use of trees in the Sandhills creates a dilemma of battling ecosystem services when there is management incentive to control the spread of woody plants for grassland diversity and forage quality and quantity.
Linking child stunting, water quality, and pathogen sharing at the human-animal-environment interface in Rwanda: A One Health study - Ben Ndayambaje
  • Dissertation Defense
  • 04/23/2023
Rwanda has made remarkable progress on many sustainable development goals (SDGs), including reducing infant, child, and maternal mortality. However, the prevalence of child stunting has remained high, impacting 33% of children under the age of five in 2020. Stunting is a complex global health challenge that can be associated with diverse individual, family, and community factors as well as environmental risk factors such as water quality. Limited clean drinking water in dry seasons and reliance on surface water sources shared with livestock and wildlife can expose people to chemical and biological contaminants. Cohabitation of people and livestock in rural areas increases the risk of zoonotic pathogen sharing and human illness. Pathogen exposure and resulting diarrheal diseases are also linked to child stunting.

To examine the relationships among stunting, water quality, and zoonotic pathogen sharing, we used primary data collected in Karongi district in western Rwanda, an area with high stunting prevalence, as well as secondary national survey data. We linked data from the 2019-2020 Rwanda Demographic and Health Survey (DHS) with national water quality testing data. We used generalized linear regression to identify demographic, socio-economic, livestock ownership, and water quality factors associated with stunting. Child factors (birth order, age, gender) and household factors (wealth status, mother’s education, rural or urban location, number of children in the household) were associated with increased stunting. Water quality (pH) was also marginally associated with stunting.

In our Karongi district field study, we paired household surveys with biological sample collection from children, livestock, and drinking water in upstream and downstream communities in the Musogoro River watershed. Water samples from surface water, public taps, and household drinking water storage containers were tested for basic physical water quality parameters, bacterial contamination (total coliform bacteria and E. coli), and potential hazardous elements (As, Cd, Cr, Cu, Fe, Pb, Mn) with public health importance. We cultured and isolated potentially zoonotic pathogens (Campylobacter, E. coli, and Salmonella) from child stool samples, livestock fecal samples, and drinking water. Surface water sources and household drinking water containers had high levels of biological (total coliform/E. coli) and chemical contaminants (iron and manganese). The prevalence of Campylobacter was high (>60%) across child, livestock, and water samples in upstream and downstream communities. High levels of E. coli were also detected across samples (>40%), with significantly higher prevalence in upstream children and livestock. Salmonella was less commonly detected, but the prevalence was significantly higher in upstream livestock. Whole genome sequencing analyses of cultured pathogens are planned to define population structures and assess pathogen sharing at the child-livestock-water interface. Our results highlight the need for further research to assess environmental factors associated with child stunting and to facilitate development of more holistic household- and watershed-based intervention strategies to improve human, animal, and ecosystem health outcomes.

The influence of landscape on exposure to and the genetic diversity of Toxoplasma gondii in felids and canids from coastal California - Kleidy Camela
  • Thesis Defense
  • 04/07/2023

Landscape composition and anthropogenic pressure can shape the transmission of pathogens between hosts in shared or adjacent habitats. Human-driven environmental changes such as urbanization can increase contact between people and animals, increasing the risk for pathogens to be transmitted at the human-domestic animal-wildlife interface. Toxoplasma gondii, a globally distributed zoonotic parasite shed by domestic and wild felids, can accumulate in terrestrial and aquatic environments leading to exposure in diverse hosts. As T. gondii can be spread through food webs (consumption of infected animal hosts) as well as by ingesting oocysts in contaminated soil, plants, and water, changes in landscape composition and configuration can impact exposure by changing contact among hosts or the potential for contacting oocysts in the environment. Although most animals and people infected with T. gondii experience mild or no clinical signs, the severity of the infection is highly dependent upon the immune system of the hosts and the strains of T. gondii involved. We used logistic regression to assess the association between landscape characteristics and T. gondii exposure as well as T. gondii genetic diversity in bobcats, coyotes, foxes and feral domestic cats from coastal California. We analyzed anthropogenic (human population density and global human footprint), demographic (sex and age), landscape composition (urbanized, agricultural, and less developed areas), and landscape configuration (diversity and evenness indices) factors as potential predictors. We found that landscape heterogeneity was positively associated with T. gondii exposure in feral cats. Animal age was consistently a strong predictor for exposure in all wild and domestic species. We also found associations between human population density and occurrence of novel atypical strains of T. gondii in wild host species. Results from this study provide insight into the ecology of T. gondii transmission among sympatric hosts in heterogeneous landscapes and highlight the need for further research to identify areas where new strains of T. gondii may emerge.

Sturgeon Chub Stats, Distributional Patterns, and Habitat Use and Benthic Fish Assemblage Structure in Missouri River Tributaries of South Dakota - Mitchell Magruder
  • Thesis Defense
  • 11/22/2022
Native species of the Missouri River drainage inhabiting benthic habitats dominate state and federal lists of species at risk. Sicklefin Chub Macrhybopsis meeki and Sturgeon Chub Macrhybopsis gelida are two native Missouri River benthic minnows that are currently under review for federal listing due to extensive population declines and local extirpations within their native range. Substantial alterations to the Missouri River in South Dakota threaten Macrhybopsis spp. and other benthic fishes; however, large, less impacted tributaries in the state may act as refugia for native species. The extent of Sicklefin Chub and Sturgeon Chub populations is largely unknown in the state and recent assessments documenting Missouri River benthic fish assemblages are lacking. Our objectives were to: 1) update the status, evaluate distributional patterns, and characterize habitat use of Sicklefin Chub and Sturgeon Chub, and 2) describe, assess, and compare patterns of benthic fish assemblage structure in the Missouri River and its major tributaries in South Dakota. Sturgeon Chub were captured in the Cheyenne, White, and Little White rivers, but were absent from the Little Missouri and Grand Rivers. Relative abundances of both age-0 and age-1+ Sturgeon Chub were highest in the White River. Distributions of Sturgeon Chubs were limited to lower areas of all rivers where stream width, turbidity, discharge, and observed habitat complexity were greatest and abundances of Flathead Chub Platygobio gracilis and Hybognathus spp. (Plains Minnow H. placitus and Western Silvery Minnow H. argyritis) were high. Sturgeon Chub primarily used main or secondary flowing channels and were predominantly found in or near the thalweg. Velocity, depth, and percent gravel predicted Sturgeon Chub presence on smaller scales. Primarily native fish assemblages were observed in all rivers. Total species richness was lowest in the White River, where extreme environmental conditions likely limit species diversity and nonnative species establishment. Longitudinal patterns of assemblage structure were observed in the White, Cheyenne, and Little White rivers due to natural and anthropogenic changes in habitat conditions. Species additions occurred as rivers gradually increased in size and habitat complexity, but species were replaced in response to abrupt habitat changes, such as those made by impoundments. High abundances of native, benthic fishes in Missouri River tributaries of South Dakota that are experiencing overall population declines highlights the importance of large, relatively unaltered tributaries to the conservation of freshwater biodiversity in North America.
A Biome in Transition: Co-production of Science for Grassland Conservation - Dillon Fogarty
  • Dissertation Defense
  • 11/21/2022
Woody plant encroachment is a global threat to the persistence of grassland biomes and the ecosystem services they support. Effective conservation in the face of biome-scale threats will require new and adaptive approaches that go beyond the disciplinary traditions of natural resource management. The focus of this dissertation is co-production of science for the conservation of grasslands threatened by woody plant encroachment. Each chapter reflects an actionable research question, co-developed between scientists and managers to better understand, and manage the underlying risks of woody encroachment. Overall, findings provide a better understanding of where grasslands are vulnerable to encroachment, key drivers of grassland vulnerability, the risk of woody encroachment to ecosystem services, and how management can reduce grassland risk to encroachment.
Different legacies, similar journeys: how factors within and outside management control structure prairie restorations and remnants - Katharine Hogan
  • Dissertation Defense
  • 11/18/2022
Ecological restoration is a critical tool in countering biodiversity collapse and can create desired positive feedbacks, i.e., native plant restoration that attracts native fauna and supports ecological function. Habitat management is critical to this process, as accelerating global change makes restored and unrestored habitat health increasingly uncertain. Because western ecological restoration is fairly new and developed alongside or after other ecology subdisciplines, there are knowledge gaps in our ability to restore at scales that can counter global degradation. Knowledge gaps include how to 1) maintain restorations over time amidst changing climate, 2) increase restoration success by partitioning controllable vs. uncontrollable factors, and 3) close the theory-application loop by using theory to predict natural systems, and natural systems to inform theory. This dissertation uses experimental and non-experimental data to investigate how factors within and outside manager control structure grassland plant communities across southern Nebraska. In a new 3-year experiment I tested the impact of seed mix (mid- and high-diversity) and haying on flowering phenology in old hay meadow restoration. Haying significantly increased flower abundance regardless of seed mix, but extra work may be needed to ensure critical spring blossoms are predominantly native, pollinator-preferred species. The non-experimental data from collaborators encompassed 19 years of sampling and 42 grassland sites (including 23 never-plowed remnants). Using those data, I explored interdecadal plant diversity in restorations, partitioned the effects of soils and management on restorations and remnants, and partitioned variance of major functional groups over five years. Plant diversity in restorations accumulated and persisted over 19 years. Soils and management impact remnant and restoration community composition, and soils also impact plant diversity. Variation within plant communities is largely stable over time, with site differences contributing most of the variance but not differing between remnants and restorations. Overall, grasslands act similarly over time regardless of restoration/remnant status and high spatial variability, and species-targeted, intensive sampling may be required to detect critical sources of variation within non-experimental plant communities.
Understanding Conservation Specialists' Role in the Adoption of Precision Agriculture in Nebraska - Morgan Register
  • Thesis Defense
  • 06/30/2022

The Delphi Method is used throughout a wide range of scientific disciplines for collecting copious amounts of qualitative data about topics of interest. However, the structure and process can be vague and variable. Within 81 peer-reviewed scientific journals, we identified 115 publications addressing the use of the Delphi Method in the fields of natural resources, fisheries, wildlife, and conservation. Our research showed that the Delphi method can successfully provide insight into a wide range of natural resources topics when reported and explained in the methodological structure. Specifically, this project used the example of the adoption of precision agriculture for conservation purposes, to display the Delphi methods abilities to address prominent questions and provide feedback that will help guide researchers to the best next steps in research and management decisions. Our research meets the demonstration of a complex issue by the need for strategic development of agricultural lands to ensure we can feed a growing world, while simultaneously reducing impacts on our natural resources such as water pollution from runoff, soil degradation, and habitat fragmentation.

To address these growing concerns, researchers are looking for ways to optimize both agricultural production and natural resource conservation. Therefore, precision conservation was developed to ensure sustainable ecosystems for future generations. Precision conservation leverages various precision agricultural tools like yield monitor data, and Geographic Information Systems (GIS). to identify areas in fields that can be diversified to optimize financial return on investment while benefiting conservation. The role conservation specialists play and their influence on the implementation and adoption of emerging precision agricultural practices remains in question. To ensure conservation specialists can clearly articulate how precision conservation can help agricultural producers feed a growing world, while simultaneously reducing impacts on our natural resources, we convened a panel of 20 conservation specialists to examine the current diverse perspectives on the progression and integration of precision agriculture in conservation management prescriptions. We used the E-Delphi method to gather data through a series of three surveys to test the hypothesis that the use of precision agriculture is becoming increasingly prevalent in the field of conservation. Our results support the hypotheses, suggesting that conservation specialists are, in fact, using precision agriculture practices in their conservation positions, but with noticeable variance in the confinements of which practices were utilized. Furthermore, the results highlight the need to provide a united message when delivering precision agriculture across varying agency and organizational platforms

An investigation of the attitudes and behavioral outcomes of Nebraskan hunters towards tick-borne disease - Dominic Cristiano
  • Thesis Defense
  • 04/19/2022

As changes in climate, land-use, and vegetation alter the landscape of the Great Plains, new threats to public health are emerging. Incidences of tick-borne disease contraction in Nebraska have increased nearly 250% over the past two decades – newly established species like Ixodes scapularis may introduce challenges for health practitioners, including more cases of Lyme disease. Strategies for tick-borne disease prevention must incorporate effective health messaging. Audience segmentation may be a useful technique to provide health communication, as it allows for targeted messaging that speaks to specific attitudes and beliefs of a given population. One tool for usefully segmenting populations is the Risk Perception Attitude Framework (RPAF) – this groups individuals into four categories based on their perceived risk towards a threat and their efficacy in protecting themselves from the threat. We applied the RPAF to a sample of hunters in Nebraska to assess differences in level of intention to perform preventative behaviors between the four RPAF groups. Our Analysis of Variance (ANOVA) model found significantly higher behavior intent among individuals in the RPAF group with highest perceived risk and self-efficacy, backing up previous RPAF literature. This information can be used to identify clusters of individuals with similar beliefs towards tick-borne disease and provide more effective health messaging about this threat.

Impacts of Anthropogenic Pressures on Ocelots (Leopardus Pardalis) in the Maya Biosphere Reserve in Guatemala - Gabriela Palomo-Munoz
  • Dissertation Defense
  • 01/14/2022
IMPACTS OF ANTHROPOGENIC PRESSURES ON OCELOTS (LEOPARDUS PARDALIS) IN THE MAYABIOSPHERE RESERVE IN GUATEMALA By Gabriela Palomo-Munoz Advisor:Andrew J Tyre and co-advisor Jeffrey Thompson January 14th 2022 9:00 CST via Zoom. Request link via DM or gabriela.palomo@huskers.unl.edu Abstract Ocelots are a Neotropical mesocarnivore distributed from the South of Texas to northern Argentina.Despite being one of the most studied mesocarnivores, there is still need to understand the effects of human activities on their populations.The Maya Biosphere Reserve (MBR) in Guatemala is a human impacted landscape that ranges from highly protected areas, sustainable forest concessions, to areas with human activities (e.g., cattle ranching,agriculture,urbanization).The MBR provides a study area in which we can investigate the anthropogenic effects on ocelots' demography and behavior. We estimated the effects of human activities on ocelot's density and space use.We also quantified the effects of forest integrity on jaguars, pumas.gray foxes, and ocelots space use, co-occurrence, and spatio-temporal patterns. Despite ocelots' tolerance to human activities, their populations heavily rely on permeable matrices surrounded by protected areas. Our results contribute to our understanding on ocelot ecology in modified landscapes.